ELISpot Flex: Mouse IL-1β (ALP)

ELISpot Flex: Mouse IL-1β (ALP)

3317-2A

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Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMTB52
Detection mAbMTB2433, biotin
Enzyme conjugateStreptavidin-ALP

In stock

Delivery 4-9 business days

Shipping $0

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ELISpot substrate: BCIP/NBT-plus for ALP


Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting mouse IL-1β using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Mouse IL-1β (ALP)
ApplicationELISpot
AnalyteIL-1β
ReactivityMouse
Specificity

This kit is based on a matched pair of mAbs specific for mouse IL-1β.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store at 4-8 °C upon receipt.

Shelf lifeAt least 18 months from date of receipt.

Mouse spleen cells (600,000 cells/well) were incubated overnight in the absence or presence of LPS + IFN-γ and the number of cells secreting IL-1β was analyzed by ELISpot.

Datasheet/Protocol
SDS

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First-ever mouse IL-1β ELISpot
Our tools for detecting mouse IL-1β: monoclonals, ELISpot and ELISA kits.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them
ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published October 14, 2022

Isolation, freezing, and thawing of splenocytes
This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.
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IL-1β

Analyte description

Interleukin 1ß (IL-1ß) is a proinflammatory cytokine and inducer of acute phase responses. IL-1ß is produced primarily by monocytes, macrophages, and dendritic cells after induction by microbes.
 

Alternative namesInterleukin-1ß, IL-1ß, IL-1F2, Interleukin-1beta, IL-1 beta, IL1b, Interleukin-1 beta
Cell typeMonocyte/MΦ, mDC
Gene ID16176